Prenatal Genetic Diagnosis of a Sex Chromosome Aneuploidy: Parent Experiences

Prenatal Genetic Diagnosis of a Sex Chromosome Aneuploidy: Parent Experiences
Sex chromosome aneuploidies (SCAs) happen in 1 in each 400 births. SCAs are extremely variable and have unsure prognoses, complicating the supply of prenatal cell-free DNA (cfDNA) outcomes or analysis following amniocentesis or chorionic villus sampling. Using a mixed-methods strategy, we explored the experiences of mother and father receiving a prenatal analysis of a fetus with SCA. Responses to open-ended questions had been qualitatively analyzed. Of the 323 mother and father who accomplished the survey, 122 obtained a prenatal analysis and answered no less than one open-ended query.
Most mother and father didn’t recall being knowledgeable that cfDNA screening or amniocentesis might reveal the presence of a SCA previous to testing and described feeling unprepared for a optimistic end result. Variation was discovered between mother and father who had been delivered a analysis by a genetic skilled versus different scientific specialties. Many mother and father expressed that the analysis was delivered in a means that emphasised the unfavorable attributes of the SCA and that they had been supplied restricted help supplies.
Parents who obtained a prenatal analysis of a SCA expressed a need for extra supportive supply of prenatal analysis that focuses on parental training and nuanced dialogue of potential phenotypes. Genetic counselors needs to be conscious of the vary of parental experiences when receiving a SCA analysis from non-genetic suppliers. Prenatal SCA diagnoses are predicted to extend as prenatal cfDNA screening turns into extra broadly used. Collaborations for higher supplier training and complete supplies on SCAs are important to facilitate the supply of SCA diagnoses and enhance guardian understanding and help.

Inference of inhabitants genetic parameters from an irregular time collection of seasonal influenza virus sequences

Basic abstract statistics that quantify the inhabitants genetic construction of influenza virus are essential for understanding and inferring the evolutionary and epidemiological processes. However, the sampling dates of international virus sequences within the final a number of a long time are scattered nonuniformly all through the calendar. Such temporal construction of samples and the small efficient measurement of viral inhabitants hampers the use of typical strategies to calculate abstract statistics.
Here, we outline statistics that overcome this downside by correcting for the sampling-time distinction in quantifying a pairwise sequence distinction. A easy linear regression technique collectively estimates the mutation price and the extent of sequence polymorphism, thus offering an estimate of the efficient inhabitants measurement. It additionally results in the definition of Wright’s FST for arbitrary time-series information. Furthermore, as a substitute for Tajima’s D statistic or the site-frequency spectrum, a mismatch distribution corrected for sampling-time variations could be obtained and in contrast between precise and simulated information.
Application of these strategies to seasonal influenza A/H3N2 viruses sampled between 1980 and 2017 and sequences simulated underneath the mannequin of recurrent optimistic choice with metapopulation dynamics allowed us to estimate the synonymous mutation price and discover parameter values for choice and demographic construction that match the remark. We discovered that the mutation charges of HA and PB1 segments earlier than 2007 had been notably excessive and that together with recurrent optimistic choice in our mannequin was important for the genealogical construction of the HA section. Methods developed right here could be typically utilized to inhabitants genetic inferences utilizing serially sampled genetic information.

Atypical Genetic Basis of Pyrazinamide Resistance in Mono-resistant Mycobacterium tuberculosis

Pyrazinamide (PZA) is a broadly used antitubercular chemotherapeutic. Typically, PZA resistance (PZA-R) emerges in M. tuberculosis strains with current resistance to isoniazid and rifampicin (MDR) and is conferred by loss-of-function pncA mutations that inhibit conversion to its energetic kind, Pyrazinoic acid (POA). PZA-R departing from this canonical situation is poorly understood. Here, we genotype pncA and purported various PZA-R genes (panD, rpsA, and clpC1) with long-read sequencing of nineteen phenotypically PZA mono-resistant isolates collected in Sweden and evaluate their phylogenetic and genomic traits to a giant set of MDR PZA-R (MDRPZA-R) isolates. We report the primary affiliation of ClpC1 mutations with PZA-R in scientific isolates, within the ClpC1 promoter (clpC1p -138) and N-terminal (ClpC1Val63Ala).
Mutations have emerged in each these areas underneath POA choice in vitro and ClpC1N-terminal has been implicated additional, by its POA-dependent efficacy in PanD proteolysis. ClpC1Val63Ala mutants spanned 4 Indo-oceanic sublineages. Indo-oceanic isolates invariably harbored ClpC1Val63Ala and had been starkly overrepresented (OR=22.2, p <0.00001) amongst PZA mono-resistant isolates (11/19) in comparison with MDRPZA-R isolates (5/80). The genetic foundation of Indo-oceanic isolates’ overrepresentation in PZA mono-resistant TB stays undetermined, however substantial circumstantial proof suggests ClpC1Val63Ala confers low-level PZA resistance. Our findings spotlight ClpC1 as doubtlessly clinically related for PZA-R and reinforce the significance of genetic background within the trajectory of resistance growth.

Exploring mother and father’ perceptions of the worth of pediatric genetic counseling affected person letters: A qualitative research presenting classes realized

Genetic counseling affected person letters are a helpful complement to genetic counseling follow. As the demand for genetic companies will increase, enhancing effectivity in each day duties resembling letter writing might enhance genetic counselor workflow. Additionally, understanding the worth recipients place on the content material of these letters previous to creating efficiencies is important towards making certain that the utility of these letters will not be misplaced. To higher perceive mother and father’ perceptions of the letter’s worth within the pediatric genetic counseling setting, we employed a qualitative design involving 13 mother and father of youngsters who obtained a affected person letter following their analysis.
Prenatal Genetic Diagnosis of a Sex Chromosome Aneuploidy: Parent Experiences
Parents participated in a semi-structured focus group, interview, or cellphone interview, and the info had been analyzed utilizing thematic evaluation. In addition to gathering perceptions of their kid’s letter, we sought to study preferences for letter size, formatting, and stage of element by asking for verbal and written suggestions on three totally different letter codecs created for a fictional affected person. We used self-determination concept (SDT) framework to create the pattern letters, which states that a person’s expertise of autonomy, competence, and relatedness can influence their capacity to interact in actions.

FD Rapid GolgiStain™ kit - Solution C

PK401-C 250 ml
EUR 185.77
Description: Best quality products

Human Apolipoprotein C-I protein control for WB

APOC11-C 100 ul
EUR 286

Human Apolipoprotein C-II protein control for WB

APOC22-C 100 ul
EUR 286

Human Apolipoprotein C-III protein control for WB

APOC32-C 100 ul
EUR 286

Purified Human C-Reactive Protein (CRP) control for WB

CRP12-C 100 ul
EUR 286

Purified Rat C-Reactive Protein (CRP) control for WB

CRP16-C 100 ul
EUR 286

Purified Dog C-Reactive Protein (CRP) control for WB

CRP18-C 100 ul
EUR 286

Recombinant (E.coli) C. perfringens beta toxin protein control for western blot

CPB12-C 100 ul
EUR 286

Recombinant (E.coli) C. perfringens epsilon toxin protein control for western blot

CPE13-C 100 ul
EUR 286

Recombinant (NS0) purified Mouse C-Reactive Protein (CRP) cotnrol for Western

CRP21-C 100 ul
EUR 286

Recombinant (NS0) purified Mouse C-Reactive Protein (CRP) cotnrol for Western

CRP24-C 100 ul
EUR 286

Recombinant (E.coli) C. perfringens alpha toxin protein control for western blot

CPA11-C 100 ul
EUR 286

Human recombinant purified His-tag c-myc protein (~65 kda) control

MYC17-C 100 ul
EUR 286

Recombinant purified Circumsporozoite (CSP, P.falciparum) C-terminal (207-397 aa) His-tag protein control for Western Blot

CSPF11-C 100 ul
EUR 286

c-Myc Oncoprotein; Clone 9E10.3 (Concentrate)

RA0226-C.1 0.1 ml
EUR 125

c-Myc Oncoprotein; Clone 9E10.3 (Concentrate)

RA0226-C.5 0.5 ml
EUR 300

c-Myc Oncoprotein; Clone MYC909 (Concentrate)

RA0227-C.1 0.1 ml
EUR 125

c-Myc Oncoprotein; Clone MYC909 (Concentrate)

RA0227-C.5 0.5 ml
EUR 300

Cytochrome c (Mitochondrial Marker); Clone 7H8.2C12 (Concentrate)

RA0359-C.1 0.1 ml
EUR 125

Cytochrome c (Mitochondrial Marker); Clone 7H8.2C12 (Concentrate)

RA0359-C.5 0.5 ml
EUR 300

Cytochrome c (Mitochondrial Marker); Clone CTC05 (Concentrate)

RA0360-C.1 0.1 ml
EUR 125

Cytochrome c (Mitochondrial Marker); Clone CTC05 (Concentrate)

RA0360-C.5 0.5 ml
EUR 300

Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA/Western

MFPM52-C 100 ul
EUR 286

Tenascin C (Stromal Marker For Epithelial Malignancy); Clone T2H5 (Concentrate)

RA0135-C.1 0.1 ml
EUR 125

Tenascin C (Stromal Marker For Epithelial Malignancy); Clone T2H5 (Concentrate)

RA0135-C.5 0.5 ml
EUR 247

Cytokeratin 8/18 (Epithelial Marker); Clone C-43 & DC10 (Concentrate)

RA0388-C.1 0.1 ml
EUR 125

Cytokeratin 8/18 (Epithelial Marker); Clone C-43 & DC10 (Concentrate)

RA0388-C.5 0.5 ml
EUR 300

HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)

RA0108-C.1 0.1 ml
EUR 125

HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)

RA0108-C.5 0.5 ml
EUR 300

CD117/c-Kit (Marker for Gastrointestinal Stromal Tumors); Clone C117/370 (Concentrate)

RA0168-C.1 0.1 ml
EUR 125

CD117/c-Kit (Marker for Gastrointestinal Stromal Tumors); Clone C117/370 (Concentrate)

RA0168-C.5 0.5 ml
EUR 300

Human C-Peptide ELISA Kit

DLR-C-Peptide-Hu-48T 48T
EUR 398
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human C-Peptide ELISA Kit

DLR-C-Peptide-Hu-96T 96T
EUR 511
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse C-Peptide ELISA Kit

DLR-C-Peptide-Mu-48T 48T
EUR 450
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse C-Peptide ELISA Kit

DLR-C-Peptide-Mu-96T 96T
EUR 582
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C-Peptide ELISA Kit

DLR-C-Peptide-Ra-48T 48T
EUR 467
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C-Peptide ELISA Kit

DLR-C-Peptide-Ra-96T 96T
EUR 605
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human C-Peptide ELISA Kit

RDR-C-Peptide-Hu-48Tests 48 Tests
EUR 404

Human C-Peptide ELISA Kit

RDR-C-Peptide-Hu-96Tests 96 Tests
EUR 556

Mouse C-Peptide ELISA Kit

RDR-C-Peptide-Mu-48Tests 48 Tests
EUR 465

Mouse C-Peptide ELISA Kit

RDR-C-Peptide-Mu-96Tests 96 Tests
EUR 643

Rat C-Peptide ELISA Kit

RDR-C-Peptide-Ra-48Tests 48 Tests
EUR 486

Rat C-Peptide ELISA Kit

RDR-C-Peptide-Ra-96Tests 96 Tests
EUR 672

Human C-Peptide ELISA Kit

RD-C-Peptide-Hu-48Tests 48 Tests
EUR 387

Human C-Peptide ELISA Kit

RD-C-Peptide-Hu-96Tests 96 Tests
EUR 532

Mouse C-Peptide ELISA Kit

RD-C-Peptide-Mu-48Tests 48 Tests
EUR 446

Mouse C-Peptide ELISA Kit

RD-C-Peptide-Mu-96Tests 96 Tests
EUR 615

Rat C-Peptide ELISA Kit

RD-C-Peptide-Ra-48Tests 48 Tests
EUR 465

Rat C-Peptide ELISA Kit

RD-C-Peptide-Ra-96Tests 96 Tests
EUR 643

DIRECTPCR LYSIS REAGENT (CELL)

302-C 100 ml
EUR 207
Description: For use with cultured cells

DIRECTPCR LYSIS REAGENT (CELL)

301-C 50 ml
EUR 141
Description: For use with cultured cells

Monkey IgM (Rhesus, non-immune control) for ELISA Kit, 1 ml (target range ~20-30 ug/ml)

7060-C 1 ml
EUR 286

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 286

Recombinant (CHO) human DNAse I Protein control for WB

DNASE12-C 100 ul
EUR 286

Human spleen lysate protein control for Deoxyribonuclease II (Dnase II)

DNASE23-C 100 ul
EUR 286

DNP-labeled Molecular weight standard proteins (21, 29, 43, 68 , and 97 kda) for Western blot

DNP45-C 1 set
EUR 286

CHIKE26-R-10

APOA11-C 100 ul
EUR 286

Apolipoprotein A-Il, Human Plasma protein control for WB

APOA21-C 100 ul
EUR 286

Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
EUR 286

Recombinant Human ApoE protein W. Blot +ve control

APOE11-C 100 ul
EUR 286

Recombinant Human ApoE protein W. Blot +ve control

APOE12-C 100 ul
EUR 286

Human plasma Apolipoprotein E protein control for WB

APOE13-C 100 ul
EUR 286

Human ApoE3 protein W. Blot +ve control

APOE31-C 100 ul
EUR 286

Recombinant purified Human ApoJ (Clusterin/Apolipoprotein J) protein control for WB

APOJ11-C 100 ul
EUR 286

Human Plasma Apolipoprotein J protein control for WB

APOJ13-C 100 ul
EUR 286

Purified Recombinant Human Angiopoietin 1 (Ang-1) protein WB +ve control

ANG12-C 100 ul
EUR 286

Purified (E.Coli) Recombinant Human Angiopoietin 2 (Ang-2) WB +ve control

ANG27-C 100 ul
EUR 286

Mouse Angiopoietin 3 (Ang-3) protein control for WB

ANG31-C 100 ul
EUR 286

Human Angiopoietin 4 (Ang-4) protein control for WB

ANG41-C 100 ul
EUR 286

Recombinant purified Human Angiogenin protein W. blot +ve control

ANGN12-C 100 ul
EUR 286

Human Angiostatin Kringles 1-3 purified protein W. blot +ve positive control

ANKR131-C 100 ul
EUR 286

Purified Human Angiostatin (Kringles 1-4) protein W. Blot +ve control

ANST12-C 100 ul
EUR 286

Human beta-2 glycoprotein (B2-GP or Apolipoprotein H/ApoH) W. Blot +ve control

B2GP11-C 100 ul
EUR 286

Purified Human beta-2 microglobulin (B2M) WB +ve control

B2M11-C 100 ul
EUR 286

Recombinant African Swine fever virus (ASFV) P30 protein control for western blot

ASFV11-C 100 ul
EUR 286

Purified, Porcine CABP9K (D9K/CALB3 or CABP1) protein control for WB

D9K12-C 100 ul
EUR 286

Recombinant Duffy Binding like5 (DBL5/VAR2CSA) Domain protein (45 kda) control for Western

DBL51-C 100 ul
EUR 286

Control/Blocking peptide for Mouse Neuronal migration protein doublecortin (DCX)

DCX11-C 100 ug
EUR 164

Recombinant Classical Swine Fever Virus E2 protein (CSFV-E2) control for western blot

CSFE21-C 100 ul
EUR 286

Recombinant Classical Swine Fever Virus Erns protein (CSFV-Erns) control for western blot

CSFR11-C 100 ul
EUR 286

Recombinant (E. coli) Circumsporozoite (P. vivax, CSP; 353-aa and GST) protein control for Western blot

CSPV11-C 100 ul
EUR 286

Human Liver Cathepsin B protein control for Western blot

CTHB11-C 100 ul
EUR 286

Human Liver Cathepsin D protein control for Western blot

CTHD11-C 100 ul
EUR 286

Recombinant purified mouse Liver Cathepsin D protein control for Western blot

CTHD12-C 100 ul
EUR 286

Human Liver Cathepsin G protein control for Western blot

CTHG11-C 100 ul
EUR 286

Human Liver Cathepsin H protein control for Western blot

CTHH11-C 100 ul
EUR 286

Human Liver Cathepsin L protein control for Western blot

CTHL11-C 100 ul
EUR 286

Recombinant purified Human Carboxyl Terminal Modulator Protein (CTMP) protein for Western blot

CTMP11-C 100 ul
EUR 286

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 286

Purified Cholera Toxin A subunit protein control for Western Blot

CTOX16-C 100 ul
EUR 286

Purified Cholera Toxin B subunit protein control for Western Blot

CTOX27-C 100 ul
EUR 286

Actin, Alpha-Smooth Muscle; Clone 1A4 (Concentrate)

A00002-C 1 ml
EUR 497
This consists of caring for a baby with particular medical wants. While the findings from this work strengthened the significance of written communication for sufferers as seen in earlier analysis, this work uncovered three main themes in regards to the letter’s worth: (a) parts resembling readability and content material influence guardian emotions of autonomy and enhance competence shifting ahead with their kid’s care; (b) mother and father worth written acknowledgment of the emotional influence of the analysis; and (c) mother and father use the letter as a instrument to speak their kid’s analysis with others. These outcomes can be utilized for creating understandable affected person letters that help autonomy, competence, and relatedness.

The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms

The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms
Nonalcoholic fatty liver illness (NAFLD) is characterised by hepatic lipid accumulation. SAMM50 encodes Sam50, a mitochondrial outer membrane protein concerned in the elimination of reactive oxygen species, mitochondrial morphology, and regulation of mitophagy. Certain single nucleotide polymorphisms (SNPs) of SAMM50 have been reported to be correlated with NAFLD.
However, the contribution of SAMM50 polymorphisms to the prevalence and severity of fatty liver in the Chinese Han cohort has not often been reported. Here, we investigated the affiliation between SAMM50 polymorphisms (rs738491 and rs2073082) and NAFLD in a Chinese Han cohort, in addition to the mechanistic foundation of this affiliation. Clinical data and blood samples had been collected from 380 NAFLD instances and 380 regular topics for the detection of genotypes and biochemical parameters. Carriers of the rs738491 T-allele or rs2073082 G-allele of SAMM50 exhibit elevated susceptibility to NAFLD (OR=1.39; 95% CI=1.14-1.71, P=0.001; OR=1.31; 95% CI=1.05-1.62, P=0.016, respectively) and are correlated with elevated serum TG, ALT, and AST ranges.
The presence of the T allele (TT+CT) of rs738491 (P<0.01) or G allele (AG+GG) of rs2073082 (P=0.03) is correlated with the severity of fatty liver in the NAFLD cohort. In vitro research indicated that SAMM50 gene polymorphisms lower its expression and SAMM50 deficiency outcomes in elevated lipid accumulation due to a lower in fatty acid oxidation. Overexpression of SAMM50 enhances fatty acid oxidation and mitigates intracellular lipid accumulation. Our outcomes affirm the affiliation between the SAMM50 rs738491 and rs2073082 polymorphisms and the danger of fatty liver in a Chinese cohort. The underlying mechanism could also be associated to decreased fatty acid oxidation attributable to SAMM50 deficiency.

Cross-species transcriptomics uncovers genes underlying genetic lodging of developmental plasticity in spadefoot toads

That hardcoded genomes can manifest as plastic phenotypes responding to environmental perturbations is a captivating function of residing organisms. How such developmental plasticity is regulated on the molecular stage is starting to be uncovered aided by the event of -omic strategies. Here, we evaluate the transcriptome-wide responses of two species of spadefoot toads with differing capability for developmental acceleration of their larvae in the face of a shared environmental danger: pond drying.
By evaluating gene expression profiles over time and performing cross-species community analyses, we recognized orthologues and purposeful gene pathways whose environmental sensitivity in expression have diverged between species. Genes associated to lipid, ldl cholesterol and steroid biosynthesis and metabolism make up most of a module of genes environmentally responsive in one species, however canalized in the opposite. The evolutionary adjustments in the regulation of the genes recognized by means of these analyses might have been key in the genetic lodging of developmental plasticity in this technique.

Development of Host-Orthogonal Genetic Systems for Synthetic Biology

The building of a host-orthogonal genetic system can’t solely decrease the affect of host-specific nuances on fine-tuning of gene expression, but in addition broaden mobile features equivalent to in vivo steady evolution of genes based mostly on an error-prone DNA polymerase. It represents an rising highly effective strategy for making biology simpler to engineer.
In this evaluation, the latest advances are described on the design of genetic methods that may be stably inherited in the host cells and are answerable for necessary organic processes together with DNA replication, RNA transcription, protein translation, and gene regulation. Their functions in artificial biology are summarized and the longer term challenges and alternatives are mentioned in growing such methods.
The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms

Investigating the inhabitants construction and genetic variety of Arabian horses in Oman utilizing SNP markers

Arabian horses had been chosen for metabolic effectivity, magnificence, effectivity and endurance. Therefore, Bedouins have for hundreds of years traced their prized horses’ ancestries. With the institution of the World Arabian Horse Organization (WAHO), registration of Arabian horses grew to become centralized and international locations worldwide registered them in its database.
Most current Arabian horses in Oman right this moment had been imported after the 1970s and are predominantly flat-racing Arabians. This work geared toward revealing the genetic background and variety of Omani Arabian horses by evaluating them with Arabian horses from a various genetic background. To that finish, we genotyped 63 randomly sampled Arabian horses from Oman utilizing the Illumina Equine SNP70. For comparability, SNP genotypes of 12 Saudi Arabian horses, 27 French, 77 Egyptian, 11 Polish and 36 US Arabians had been included in the examine. We moreover included 17 Thoroughbred horses and 21 horses representing giant and small breeds as an outgroup. Our MDS evaluation and phylogenetic evaluation confirmed that the Arabian horses in Oman cluster primarily with French Arabian horses, with a number of horses clustering inside the Polish/US Arabians.
The French Arabian horse cluster was the closest to the Thoroughbred horses. Amongst the Arabian horses, plink common genomic inbreeding ranges had been highest in the Egyptian Arabian (0.169) adopted by the Saudi Arabian horses (0.137) and lowest in the Omani and French Arabian horses, -0.041 and -0.079 respectively. To our data, that is the primary report on the genetic background and variety of Arabian horses in Oman. Our outcomes demonstrated a particular subpopulation construction amongst Arabian horses and this data ought to advise future decision-making on Arabian horse breeding.

A generalized sturdy allele-based genetic affiliation check

The allele-based affiliation check, evaluating allele frequency distinction between case and management teams, is regionally strongest. However, utility of the classical allelic check is restricted in apply, as a result of the tactic is delicate to the Hardy-Weinberg equilibrium (HWE) assumption, not relevant to steady traits, and never straightforward to account for covariate impact or pattern correlation.
To develop a generalized sturdy allelic check, we suggest a brand new allele-based regression mannequin with particular person allele because the response variable. We present that the rating check statistic derived from this sturdy and unifying regression framework incorporates a correction issue that explicitly adjusts for potential departure from HWE, and encompasses the classical allelic check as a particular case.
When the trait of curiosity is steady, the corresponding allelic check evaluates a weighted distinction between individual-level allele frequency estimate and pattern estimate the place the load is proportional to a person’s trait worth, and the check stays legitimate beneath Y-dependent sampling. Finally, the proposed allele-based technique can analyze a number of (steady or binary) phenotypes concurrently and multi-allelic genetic markers, whereas accounting for covariate impact, pattern correlation and inhabitants heterogeneity. To assist our analytical findings, we offer empirical proof from each simulation and utility research. This article is protected by copyright. All rights reserved.

PHACTR1 genetic variability is not critical in small vessel ischemic disease patients and PcomA recruitment in C57BL/6J mice

PHACTR1 genetic variability is not critical in small vessel ischemic disease patients and PcomA recruitment in C57BL/6J mice
Recently, a number of genome-wide affiliation research recognized PHACTR1 as key locus for 5 numerous vascular issues: coronary artery disease, migraine, fibromuscular dysplasia, cervical artery dissection and hypertension. Although these signify important threat components or comorbidities for ischemic stroke, PHACTR1 function in mind small vessel ischemic disease and ischemic stroke most vital survival mechanism, such because the recruitment of mind collateral arteries like posterior speaking arteries (PcomAs), stays unknown.
Therefore, we utilized exome and genome sequencing in a multi-ethnic cohort of 180 early-onset unbiased familial and apparently sporadic mind small vessel ischemic disease and CADASIL-like Caucasian patients from US, Portugal, Finland, Serbia and Turkey and in 2 C57BL/6J stroke mouse fashions (bilateral widespread carotid artery stenosis [BCCAS] and center cerebral artery occlusion [MCAO]), characterised by totally different levels of PcomAs patency. We report three very uncommon coding variants in the small vessel ischemic disease-CADASIL-like cohort (p.Glu198Gln, p.Arg204Gly, p.Val251Leu) and a stop-gain mutation (p.Gln273*) in one MCAO mouse.
These coding variants do not cluster in PHACTR1 identified pathogenic domains and are not prone to play a critical function in small vessel ischemic disease or mind collateral circulation. We additionally exclude the chance that replicate quantity variants (CNVs) or a variant enrichment in Phactr1 could also be related to PcomA recruitment in BCCAS mice or linked to numerous vascular traits (cerebral blood move pre-surgery, PcomA measurement, leptomeningeal microcollateral size and junction density throughout mind hypoperfusion) in C57BL/6J mice, respectively.
Genetic variability in PHACTR1 is not prone to be a standard susceptibility issue influencing small vessel ischemic disease in patients and PcomA recruitment in C57BL/6J mice. Nonetheless, uncommon variants in PHACTR1 RPEL domains could affect the stroke final result and are price investigating in a bigger cohort of small vessel ischemic disease patients, totally different ischemic stroke subtypes and with purposeful research.

Insight of fetal to grownup hemoglobin change: Genetic modulators and therapeutic targets

The medical heterogeneity of β-hemoglobinopathies is so variable that it prompted the researchers to determine the genetic modulators of those ailments. Though the first modulator is the kind of β-globin mutation which impacts the diploma of β-globin chain synthesis, the co-inheritance of α-thalassemia and the fetal hemoglobin (HbF) ranges additionally act as potent secondary genetic modifiers.
As elevated HbF ranges ameliorate the severity of hemoglobinopathies, in this evaluate, the genetic modulators mendacity inside and outdoors the β-globin gene cluster with their believable function in governing the HbF ranges have been summarised, which in future could act as potential therapeutic targets.
PHACTR1 genetic variability is not critical in small vessel ischemic disease patients and PcomA recruitment in C57BL/6J mice

Genetic affiliation of MMP14 promoter variants and their purposeful significance in gallbladder most cancers pathogenesis

Gallbladder most cancers (GBC) is comparatively uncommon however exhibits excessive frequency in sure geographical areas and ethnic teams, which embrace Northern and Eastern states of India. Previous research in India have indicated the doable function of genetic predisposition in GBC pathogenesis. Although matrix metalloproteinase-14 (MMP14) is identified modulator of tumour microenvironment and tumorigenesis and TCGA information additionally suggests its upregulation but, its function in genetic predisposition for GBC is utterly unknown.
We explored MMP14 promoter genetic variants as threat components and their implication in expression modulation and the pathogenesis of GBC. We genotyped all single nucleotide polymorphisms of MMP14 promoter by Sanger’s sequencing in roughly 300 GBC and 300 management examine topics of Indian ethnicity and, in 26 GBC tissue samples. Protein expression of MMP14 in GBC tissue samples was checked by immunohistochemistry. In vitro luciferase reporter assay was carried out to elucidate function of promoter genetic variants on expression ranges in two totally different cell traces.
MMP14 promoter variants, rs1003349 (p worth = 0.0008) and rs1004030 (p worth = 0.0001) have been considerably related to GBC. Luciferase reporter assay confirmed excessive expression for threat alleles of each the SNPs. Genotype-phenotype correlation for rs1003349 and rs1004030, in affected person pattern, confirmed that threat allele carriers had increased expression ranges of MMP14; furthermore, the correlation sample matched with genetic affiliation fashions. Overall, this examine unravels the affiliation of MMP14 promoter SNPs with GBC which contribute to pathogenesis by rising its expression.

Compact Genetic Algorithm-based Feature Selection for Sequence-based Prediction of Dengue-Human Protein Interactions

Dengue Virus (DENV) an infection is one of many quickly spreading mosquito-borne viral infections in people. Every yr, round 50 million folks get affected by DENV an infection, ensuing in 20,000 deaths. Despite the latest experiments specializing in dengue an infection to know its performance in the human physique, a number of functionally vital DENV-human protein-protein interactions (PPIs) have remained unrecognized. This article presents a mannequin for predicting new DENV-human PPIs by combining totally different sequence-based options of human and dengue proteins just like the amino acid composition, dipeptide composition, conjoint triad, pseudo amino acid composition, and pairwise sequence similarity between dengue and human proteins.
A Learning vector quantization (LVQ)-based Compact Genetic Algorithm (CGA) mannequin is proposed for characteristic subset choice. CGA is a probabilistic approach that simulates the habits of a Genetic Algorithm (GA) with lesser reminiscence and time necessities. Prediction of DENV-human PPIs is carried out by the weighted Random Forest approach because it is discovered to carry out higher than different classifiers. We have predicted 1013 PPIs between 335 human proteins and 10 dengue proteins.

5-50UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-50 1/pk
EUR 425
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

DiscoveryPak™ Antiviral Agents Set

S252-8 8 antiviral agents
EUR 839

ECM antibody

70R-12136 100 ug
EUR 403
Description: Rabbit polyclonal ECM antibody

ECM Antibody

3843-100
EUR 316

ECM Antibody

3843-30T
EUR 146

1730 8 SNAP-SEAL 8 OZ

1730-8 100/pk
EUR 74
Description: Disposable Plastic; Plastic Containers

ExpressMax™ Formula 8

144 500 g
EUR 102

Optional eight channel adapter

V1002-8 1 PC
EUR 236.39

AXYPET 0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-10-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-10-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 20-200UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-200-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 20-200UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-200-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 50-300UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-300-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 50-300UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-300-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 5-50UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-50-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 5-50UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-50-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

ECM Blocking Peptide

33R-10952 50 ug
EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ECM antibody, catalog no. 70R-12136

ECM Blocking Peptide

3843BP-50
EUR 153

Polyclonal ECM Antibody

APR00211G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ECM . This antibody is tested and proven to work in the following applications:

Individual Reaction Mix 8

G065-8 200 reactions
EUR 167

Human IL-8 Recombinant Protein

R00423-8 5ug/vial
EUR 259
Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast.

AXYGEN® AXYPET® PRO 0.5-10 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE

AP-8-10-P 1/pk
EUR 455
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYGEN® AXYPET® PRO 20-200 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE

AP-8-200-P 1/pk
EUR 455
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYGEN® AXYPET® PRO 30-300 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE

AP-8-300-P 1/pk
EUR 455
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYGEN® AXYPET® PRO 5-50 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE

AP-8-50-P 1/pk
EUR 455
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

CytoSelect 24-well Cell Migration Assay (8 ?m), Colorimetric

CBA-100 12 assays
EUR 543
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Colorimetric

CBA-100-5 5 x 12 assays
EUR 2288
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Fluorometric

CBA-101 12 assays
EUR 554
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Fluorometric

CBA-101-5 5 x 12 assays
EUR 2323
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106-5 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

WB ECM kit (Mouse IgG)

LF-QC5001 1 kit
EUR 253
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Rabbit IgG)

LF-QC5002 1 kit
EUR 253
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Goat IgG)

LF-QC5003 1 kit
EUR 253
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Rat IgG)

LF-QC5004 1 kit
EUR 253
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Mouse IgM)

LF-QC5005 1 kit
EUR 253
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

DLR-8-OHdG-Ge-48T 48T
EUR 469
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.

8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

DLR-8-OHdG-Ge-96T 96T
EUR 608
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RD-8-OHdG-Ge-48Tests 48 Tests
EUR 467

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RD-8-OHdG-Ge-96Tests 96 Tests
EUR 646

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RDR-8-OHdG-Ge-48Tests 48 Tests
EUR 488

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RDR-8-OHdG-Ge-96Tests 96 Tests
EUR 676

pAAV-DJ/8 Vector

VPK-420-DJ-8 10 µg
EUR 647
Description: The pAAV-DJ/8 vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ/8. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ/8 packaging.

ADAPTOR, 8 CHANNEL,1 EA

4931 1/pk
EUR 186
Description: Corning Liquid Handling Equipment; Stripettors

CytoSelect 24-well Cell Haptotaxis Assay (8 µm), COL-coated, Colorimetric

CBA-100-COL 12 assays
EUR 566
Description: Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix. The CytoSelect Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 µm membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.

CytoSelect 24-well Cell Haptotaxis Assay (8 µm), FN-coated, Colorimetric

CBA-100-FN 12 assays
EUR 566
Description: Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix. The CytoSelect Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 µm membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.

CytoSelect 24-well Cell Haptotaxis Assay (8 µm), COL-coated, Fluorometric

CBA-101-COL 12 assays
EUR 595
Description: Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix. The CytoSelect Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 µm membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.

CytoSelect 24-well Cell Haptotaxis Assay (8 µm), FN-coated, Fluorometric

CBA-101-FN 12 assays
EUR 595
Description: Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix. The CytoSelect Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 µm membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.

CytoSelect Leukocyte Transmigration Assay

CBA-212 24 assays
EUR 740
Description: Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. It is the final step in a cascade of interactions between cells and the endothelium. CytoSelect Leukocyte Transmigration Assay provides a robust system for the quantitative determination of transmigrations and interactions between endothelium and leukocytes. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with 3 µm pore size membrane inserts.

PSA (Prostate-specific antigen) ELISA test

8 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen)

CytoSelect 24-Well Cell Migration Assay (8 µm, Colorimetric Format), Trial Size

CBA-100-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (8 µm, Fluorometric Format), Trial Size

CBA-101-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

Transmembrane Channel Like 8 (TMC8) Antibody

abx027907-400ul 400 ul
EUR 523

Transmembrane Channel Like 8 (TMC8) Antibody

abx027907-80l 80 µl
EUR 286

Transmembrane Channel Like 8 (TMC8) Antibody

20-abx219015
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Transmembrane Channel Like 8 (TMC8) Antibody

20-abx329114
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

BindPro™

BP355-15 15mL
EUR 481

BindPro™

BP355-50 50mL
EUR 806

HemogloBind™

H0145-05 5 mL
EUR 359
Description: Hemoglobin Removal Kit

HemogloBind™

H0145-15 15 mL
EUR 709
Description: Hemoglobin Removal Kit

HemogloBind™

H0145-50 50 mL
EUR 1420
Description: Hemoglobin Removal Kit

ProCipitate™

P0050-100 100 mL
EUR 1217
Description: DNA/RNA Enrichment Kit

ProCipitate™

P0050-30 30 mL
EUR 511
Description: DNA/RNA Enrichment Kit

MycoRid™

M093-10ML 10mL
EUR 101

MycoRid™

M093-1ML 1mL
EUR 30

MycoRid™

M093-5x10ML 5x10mL
EUR 340

MycoRid™

M093-5x1ML 5x1mL
EUR 74

Cleanascite™

X2555-10 10 mL
EUR 405
Description: Lipid Removal Reagent

Cleanascite™

X2555-100 100 mL
EUR 704
Description: Lipid Removal Reagent

Cleanascite™

X2555-1000 1 Liter Ask for price
Description: Lipid Removal Reagent

Cleanascite™

X2555-50 50 mL
EUR 521
Description: Lipid Removal Reagent

Viraffinity™

V1062-15 15 mL
EUR 501

EmbryoFix™

PF102 20 ml
EUR 54.5
Description: Best quality products

CytoSelect Tumor-endothelium Adhesion Assay

CBA-215 100 assays
EUR 577
Description: Leukocyte or tumor cell interactions with vascular endothelium consist of a cascade of processes including the firm attachment of cells to endothelial cell adhesion molecules. The CytoSelect Tumor Endothelium Adhesion Assay provides a robust system for the quantitative determination of interactions between tumor cells and endothelium. Adherent cells can be easily quantified on a fluorescence plate reader.

CytoSelect Tumor Transendothelial Migration Assay

CBA-216 24 assays
EUR 740
Description: Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. It is the final step in a cascade of interactions between cells and the endothelium. CytoSelect Tumor Transendothelial Migration Assay provides a robust system for the quantitative determination of transmigrations and interactions between endothelium and tumor cells. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with 8 µm pore size membrane inserts.

CytoSelect LDH Cytotoxicity Assay Kit

CBA-241 960 assays
EUR 403
Description: Cell Biolabs? CytoSelect LDH Cytotoxicity Assay Kit provides a colorimetric format for measuring and monitoring cell cytotoxicity.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates.  Cells can be plated and then treated with compounds or agents that affect cell viability.  Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media.  The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent.  In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH).   The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form.  An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels.  The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect BrdU Competitive ELISA Kit

CBA-5098 96 assays
EUR 543

CytoSelect IdU Competitive ELISA Kit

CBA-5100 96 assays
EUR 543

CytoSelect EdU Competitive ELISA Kit

CBA-5101 96 assays
EUR 543

AAV-DJ/8 Helper Free Packaging System

VPK-400-DJ-8 1 kit
EUR 972
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-DJ/8 Helper Free Expression System

VPK-410-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

scAAV-DJ/8 Helper Free Expression System

VPK-430-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

ASPIR-8, 5ml reservoir for 8-channel pipettes, individually wrapped

P7005-1S 100/pack
EUR 99.95
Description: ASPIR-8 5ml reservoir for 8-channel pipettes

ASPIR-8, 25ml reservoir for 8-channel pipettes, individually wrapped

P7025-1S 100/pack
EUR 99.95
Description: ASPIR-8 25ml reservoir for 8-channel pipettes

ASPIR-8, 10ml reservoir for 8-channel pipettes, individually wrapped

P8010-1S 100/pack
EUR 99.95
Description: ASPIR-8 10ml reservoir for 8-channel pipettes

CytoSelect 24-well Cell Migration and Invasion Assay (8 µm), Colorimetric, Combo Kit

CBA-100-C 2 x 12 assays
EUR 972
Description: If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits. Each 24-well combo kit provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays.

CytoSelect 24-well Cell Migration and Invasion Assay (8 µm), Colorimetric, Combo Kit

CBA-100-C-5 10 x 12 assays
EUR 3976
Description: If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits. Each 24-well combo kit provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays.

CytoSelect 24-well Cell Migration and Invasion Assay (8 µm), Fluorometric, Combo Kit

CBA-101-C 2 x 12 assays
EUR 972
Description: If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits. Each 24-well combo kit provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays.

CytoSelect 96-well Cell Migration and Invasion Assay (8 µm), Fluorometric, Combo Kit

CBA-106-C 2 x 96 assays
EUR 1146
Description: If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits. Each 96-well combo kit provides sufficient reagents to perform 96 cell migration plus 96 cell invasion assays.

AAV-DJ/8 Helper Free Promoterless Expression System

VPK-411-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

PIPETTOR,8 CHANNEL,20-200UL,1 EA

4888 1/pk
EUR 849
Description: Corning Liquid Handling Equipment; Costar 8 Pette and 12 Pette Multichannel Pipettors
All predicted interactions are validated by literature filtering, GO-based evaluation, and KEGG Pathway enrichment evaluation. This examine will encourage the identification of potential targets for simpler anti-dengue drug discovery.